Definition
Molecules seperate with terrible pressure High performance liguid chromatography. High performance liguid chromatography due to working with high pressure. The polarity base is the separation of molecules.
Components : Normal Phase And Reverse Phase
| Analyte | Soluble sample | Soluble sample |
| Stationary Phase | Polar Hydrophilic Liquid / Solid ( Silica ) |
Non Polar Hydrophobic Ligid / Solid.
( Silica Coasted with long Chains of hydrocarbons ). |
| Mobile Phase | Nonpolar Liquid | Polar Liquid ( Water / Methanol ) |
Principle :
- Polar molecue dont react with non polar molecule.
- Non Polar molecule react with non polar molecule.
Many peak available is the chromatogram. There are two types, Qualitative analysis and Quantitative analysis.
Qualitative Analysis
Qualitative analysis is the discovery of how many molecules a sample has on hand.
Quantitative Analysis
Quantitative analysis is the act of knowing how much there is.
Advantages :
- Efficient in analysis biological samples such proteins , lipids etc.
- Fast, sensitive, automated and precise.
- Both Qualitative and Quantitative analysis can be done.
Disadvantages :
- Expensive
- Detector is complex.
- Difficult for novices ( need skill to handle ).
